L5263

Sigma

 

Lyticase from Arthrobacter luteus

partially purified powder, ≥2,000 units/mg protein

CAS Number:37340-57-1
MDL number:MFCD00131560

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Description

Biochem/physiol ActionsLyticase hydrolyzes poly-β(1→3)-glucose such as yeast cell wall glucan.
Other NotesView more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer
Unit DefinitionOne unit will produce a ΔA800 of 0.001 per min at pH 7.5 at 25 °C, using a suspension of yeast as substrate in a 3 mL reaction mixture.
Physical formPartially purified powder containing ammonium sulfate and stabilizer
ApplicationYeast cells are difficult to disrupt because the cell walls may form capsules or resistant spores. DNA can be extracted from yeast by using lysing enzymes such as lyticase, chitinase, zymolase, and gluculase to induce partial spheroplast formation; spheroplasts are subsequently lysed to release DNA. Lyticase is preferred to digest cell walls of yeast and generate spheroplasts from fungi for transformation.
Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.

Properties

formpartially purified powder
compositionProtein, ≥10% biuret
storage temp.2-8°C

Safety

Personal Protective EquipmentEyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter
Hazard CodesXn
Risk Statements42
Safety Statements22
WGK Germany3

References

referenceBir, N., et al. Prep. Biochem. 25, 171-181, (1995)
 van Burik, J.A., et al. Med. Mycol 36, 299-303, (1998)
 Jazwinski, S.M. Meth. Enzymol. 182, 154-174, (1990)
 Phalip, V., et al. Biotechnol. Lett. 26, 409-413 , (2004)